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Tissue culture

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Title: Tissue culture  
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Subject: Cell culture, Nepenthes rajah, Organ culture, Tamara Awerbuch-Friedlander, Biotechnology
Collection: Cell Culture, Histology, Laboratory Techniques
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Tissue culture

Flasks containing tissue culture growth medium which provides nourishment to growing cells.

Tissue culture is the growth of growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows, M.D.[1]


  • Historical usage 1
  • Modern usage 2
  • See also 3
  • References 4
  • External links 5

Historical usage

In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue.[2] In 1996, the first use of regenerative tissue was used to replace a small distance of a urethra, which led to the understanding that the technique of obtaining samples of tissue, growing it outside the body without a scaffold, and reapplying it, can be used for only small distances of less than 1 cm. [3]

Modern usage

Cultured cells growing in growth medium

In modern usage, tissue culture generally refers to the growth of cells from a tissue from a immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [4]The term tissue culture is often used interchangeably with cell culture

The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture.

Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed.

Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium[5]

See also


  1. ^ Carrel, Alexis and Montrose T. Burrows “Cultivation of Tissues in Vitro and its Technique”; Journal of Experimental Medicine 13 (1911: 387-96)
  2. ^ Steinhardt, E; Israeli, C; and Lambert, R.A. (1913) "Studies on the cultivation of the virus of vaccinia" J. Inf Dis. 13, 294–300
  3. ^ TEDTalks. (2010, January 21). Anthony Atala on growing organs [video file]. Retrieved May 15, 2011 from ( engineering_tissue.html).
  4. ^ Martin, Bernice M. "Routine Cell Culture." Tissue Culture Techniques: An Introduction. Boston: Birkhäuser, 1994. 29-30. Print.
  5. ^ Reece, Jane B., Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, and Robert B. Jackson. "Angiosperm Reproduction and Biotechnology." Campbell Biology. 9th ed. San Francisco, CA: Benjamin Cummings, 2011. 860. Print.

External links

  • - how-to guide for biology students
  • Plant Tissue Culture - way to "xerox" a plant
  • CELOS - a division of CELOS (Center for Agricultural Research in Suriname)
  • Cell Culture Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique including basic cell culture protocols and video training
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